Secale cereale

To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. Our study indicated that cryopreservation slowed seed deterioration as evidenced by higher germination rates compared to conventional storage, had only minimal effects on other phenotypic traits, and had no significant effects on DNA methylation status.

Secale cereale

In this study, we used the ELISA method to initially determine whether 5hmC is present in the DNA of four rye species: Secale cereale, Secale strictum, Secale sylvestre, and Secale vavilovii. The result of the ELISA test was positive; thus, we performed a detailed analysis of the global level of 5hmC, 5fC, 5caC, and 5hmU (in the form of deoxynucleosides) by MS in the caryopses, coleoptiles, roots, leaves, and stems in all these species.

Secale cereale

the analysis of genomic methylation by genomic sequencing was probably not influenced by PCR bias. We therefore assumed that the data from genomic sequencing could be quantified, thus allowing comparisons between the different species.

Secale cereale

We analysed the chromatin properties of both types of nuclei after first and second pollen mitosis in rye (Secale cereale).

Secale cereale

The main objective of the present study was to investigate changes in the DNA stability and cytosine methylation status during seed storage and ageing. Genetic stability was assessed with Random Amplified Polymorphic DNA (RAPD) technique, and epigenetic changes were studied using Methylation Sensitive Amplified Polymorphism (MSAP) markers. Non-stored and stored rye seeds at different stages of ageing were compared. Also, seedlings obtained from stored seeds were compared to seedlings obtained from control (non-stored) seeds.

Secale cereale

We analysed thechromatin properties of both types of nuclei after firstand second pollen mitosis in rye (Secale cereale). Thecondensed chromatin of generativenuclei is ear- marked by an enhanced level of histone H3K4/K9dimethylation and H3K9 acetylation. Differences between rye and A. thaliana data suggestthat the chromatin organization in mature nuclei ofpollen grains is not universal across angiosperms.

Secale cereale

In order to clarify the relationship between chromosomecondensation and histone modifications in different plantspecies, the present study analyzes the chromosomal dis-tribution of selected histone H3 modifications associatedwith transcriptional regulation and differentiation ofeuchromatin and heterochromatin (e.g. acetylation, phos-phorylation, and methylation of diverse residues)throughout all phases of both meiosis and mitosis.

Secale cereale

In this study, through in situ localization of rye B-specific repetitive DNA families D1100 and E3900, we disclose that the B heterochromatic domain is consistently decondensed at interphase in two distinct genomic backgrounds of rye and also in a wheat(Triticum aestivum)–rye B addition line.