Avena sativa

Metabolic gene clusters in the model plant species Arabidopsis thaliana are delineated by blocks of two different types of chromatin marks, histone H3 lysine 27 trimethylation (associated with cluster repression) and histone variant H2A.Z (associated with cluster activation) and that these features can be exploited in genome-wide mining approaches for cluster discovery. We further show that cluster-specific chromatin modifications mark metabolic gene clusters not only in A. thaliana but also in oat and maize.

Avena sativa

We report the first comprehensive study of the SPL/miR156 regulatory hub in oat in which 28 SPLs (AsSPLs) and 21 novel precursors of miR156 (AsmiR156) were identified.

Secale cereale

This study compared the level of methylation of cytosines on a global (ELISA) and genomic scale (MSAP) between the species of the genus Secale. We analyzed whether the interspecific variation of cytosine methylation was associated with the size of the genome (C-value) and the content of telomeric heterochromatin.

Secale cereale

To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. Our study indicated that cryopreservation slowed seed deterioration as evidenced by higher germination rates compared to conventional storage, had only minimal effects on other phenotypic traits, and had no significant effects on DNA methylation status.

Secale cereale

In this study, we used the ELISA method to initially determine whether 5hmC is present in the DNA of four rye species: Secale cereale, Secale strictum, Secale sylvestre, and Secale vavilovii. The result of the ELISA test was positive; thus, we performed a detailed analysis of the global level of 5hmC, 5fC, 5caC, and 5hmU (in the form of deoxynucleosides) by MS in the caryopses, coleoptiles, roots, leaves, and stems in all these species.

Secale cereale

the analysis of genomic methylation by genomic sequencing was probably not influenced by PCR bias. We therefore assumed that the data from genomic sequencing could be quantified, thus allowing comparisons between the different species.

Secale cereale

We analysed the chromatin properties of both types of nuclei after first and second pollen mitosis in rye (Secale cereale).

Secale cereale

The main objective of the present study was to investigate changes in the DNA stability and cytosine methylation status during seed storage and ageing. Genetic stability was assessed with Random Amplified Polymorphic DNA (RAPD) technique, and epigenetic changes were studied using Methylation Sensitive Amplified Polymorphism (MSAP) markers. Non-stored and stored rye seeds at different stages of ageing were compared. Also, seedlings obtained from stored seeds were compared to seedlings obtained from control (non-stored) seeds.