Triticale

The study evaluated relationships between DNA methylation, changes in DNA sequence variation, and green plant regeneration efficiency influenced by copper and silver ions during triticale plant regeneration.

Triticale

we employed structural equation modeling to evaluate the relationship between de novo DNA methylation affecting the asymmetric context of CHH sequences, the methylation-sensitive Amplified Fragment Length Polymorphism related sequence variation, and the concentration of Cu(II) and Ag(I) ions in induction media, as well as their effect on S-adenosyl-L-methionine perturbations, observed using FTIR spectroscopy, and the green plant regeneration efficiency.

Triticale

The obtained low sequencing depth was sufficient for various tasks including detection of InDels and single-nucleotide variations (SNPs), read phasing and methylation profiling. Using nCATS data, we demonstrated that glutenin genes possess gene-body methylation with hypermethylated CDS part and hypomethylated promoter regions.

Triticale

Our relative quantitative MSAP approach, based on methylation events affecting cytosines within HpaII–MspI recognition sequences, was capable of quantifying de novo methylation, demethylation, methylation, and non-methylated status in control and stressed Al-tolerant and non-tolerant triticale inbred lines.

Triticale

A structural equation model reflecting complex relationships between sequence variation, de novo DNA methylation within asymmetric sequence contexts, and copper ions in the IM, as well as, GSH, and GPRE, was evaluated.

Triticale

The amounts of pectin demethylation, SAM, de novo methylation, and GSH are connected in the model to explain GPRE. By altering the concentration of Cu(II) ions in the medium, which influences the amount of pectin, triticale’s GPRE can be increased.

Triticale

We present the development of the theoretical background of the metAFLP approach which allows for partition of complex variation into sequence changes, de novo methylation and demethylation of the regenerants derived via in vitro tissue culture methods in the case of triticale.

Triticale

The aim of this study was to use metAFLP to quantify TCIV in several triticale donor-regenerant sets developed using different androgenesis and somatic embryogenesis processes.